anti phosphorylated p65 ser276 antibody  (Bioss)

Journal: Journal of Nanobiotechnology

Article Title: An orally-administered nanotherapeutics with gold nanospheres supplying for rheumatoid arthritis therapy by re-shaping gut microbial tryptophan metabolism

doi: 10.1186/s12951-025-03450-7

Figure Lengend Snippet: IPA/IAA mixture significantly inhibited NF-κB signaling pathway via the upregulation of PTEN. A PCA analysis showed distinct clustering between IPA/IAA-treated and control groups. B Heatmap of Differentially expressed genes (DEGs) in the IPA + IAA group compared to the control group. Different colors represent different levels of gene relative expression, ranging from blue through white to red, indicating expression levels that range from low to high. Red indicates highly expressed genes, and blue shows lowly expressed genes. C Bar plot of DEGs between IPA + IAA and CT groups (|fold change|> 1.35, p < 0.05). D and E Positive regulation of cell migration (enrichment score = − 0.48) and focal adhesion (enrichment score = − 0.47) pathways were down-regulated in the IPA/IAA group compared to the CT group as validated by GSEA. F KEGG enrichment bar plot. The y-axis represents the names of the top 20 KEGG pathways with the smallest p-values, and the x-axis represents the -log10 value of the p-value from the KEGG pathway enrichment analysis. G PI3K/AKT pathway (enrichment score = − 0.38) was down-regulated in IPA/IAA as validated by GSEA. H Volcano plot of DEGs in the IPA + IAA group compared to the CT group. PTEN was significantly upregulated in the IPA + IAA group. I The relative expression of PTEN between CT and IPA + IAA groups. J Relative expression of p-p65, p65, p-IκBα, IκBα, p-PTEN, and PTEN in the presence or absence of IPA/IAA mixture during the stimulation for 0–120 min at the protein level. β-actin was used as an internal control. K–P Quantitative analyses of p-p65, p65, p-IκBα, IκBα, p-PTEN, and PTEN expression. Q Representative images of the intracellular location of the NF-κB p65. R MH7A cells were cultured with or without the IPA/IAA mixture for 12 h and treated with cycloheximide (CHX) (50 μg/mL). Total cell lysates were subjected to immunoblotting analysis. S Quantitative analyses of PTEN expression treated with CHX in the presence or absence of IPA/IAA mixture (0, 4, 8, 12 h). T MH7A cells were pretreated with IPA/IAA mixture for 8 h, with or without MG132 (50 μM) for 2 h. The cell lysates were immunoprecipitated with PTEN antibody and immunoblotted with ubiquitin and PTEN antibodies. U–V Relative expression levels of ubiquitin in PTEN

Article Snippet: Paw tissue sections were blocked with 5% BSA and incubated overnight at 4 °C with primary antibodies: anti-PTEN (Cat#: 60,300–1, Proteintech, 1:200), anti-p-p65 (Cat#: bs-3543R, Bioss, 1:200).

Techniques: Control, Expressing, Migration, Cell Culture, Western Blot, Immunoprecipitation, Ubiquitin Proteomics

Journal: Journal of Nanobiotechnology

Article Title: An orally-administered nanotherapeutics with gold nanospheres supplying for rheumatoid arthritis therapy by re-shaping gut microbial tryptophan metabolism

doi: 10.1186/s12951-025-03450-7

Figure Lengend Snippet: The IPA/IAA mixture exerted significant anti-inflammatory and protective effects in CIA mice. A Arthritis severity scores in CIA mice treated with the IPA/IAA mixture or normal saline. B Representative histological images of joint sections stained with H&E. C Representative TRAP-stained images of joint sections in CIA mice. D Representative photographs and micro-CT images of paws. E and F Representative immunohistochemical images showing expression of p-p65 and PTEN in fibroblast-like synoviocytes. G Histological scores of joints. H Quantitative analyses of TRAP staining. I Quantitative analyses of bone histomorphometry indexes. J and K The Average Optical Density (AOD) of p-p65 and PTEN

Article Snippet: Paw tissue sections were blocked with 5% BSA and incubated overnight at 4 °C with primary antibodies: anti-PTEN (Cat#: 60,300–1, Proteintech, 1:200), anti-p-p65 (Cat#: bs-3543R, Bioss, 1:200).

Techniques: Saline, Staining, Micro-CT, Immunohistochemical staining, Expressing

Journal: Journal of Nanobiotechnology

Article Title: An orally-administered nanotherapeutics with gold nanospheres supplying for rheumatoid arthritis therapy by re-shaping gut microbial tryptophan metabolism

doi: 10.1186/s12951-025-03450-7

Figure Lengend Snippet: IPA/IAA mixture significantly inhibited NF-κB signaling pathway via the upregulation of PTEN. A PCA analysis showed distinct clustering between IPA/IAA-treated and control groups. B Heatmap of Differentially expressed genes (DEGs) in the IPA + IAA group compared to the control group. Different colors represent different levels of gene relative expression, ranging from blue through white to red, indicating expression levels that range from low to high. Red indicates highly expressed genes, and blue shows lowly expressed genes. C Bar plot of DEGs between IPA + IAA and CT groups (|fold change|> 1.35, p < 0.05). D and E Positive regulation of cell migration (enrichment score = − 0.48) and focal adhesion (enrichment score = − 0.47) pathways were down-regulated in the IPA/IAA group compared to the CT group as validated by GSEA. F KEGG enrichment bar plot. The y-axis represents the names of the top 20 KEGG pathways with the smallest p-values, and the x-axis represents the -log10 value of the p-value from the KEGG pathway enrichment analysis. G PI3K/AKT pathway (enrichment score = − 0.38) was down-regulated in IPA/IAA as validated by GSEA. H Volcano plot of DEGs in the IPA + IAA group compared to the CT group. PTEN was significantly upregulated in the IPA + IAA group. I The relative expression of PTEN between CT and IPA + IAA groups. J Relative expression of p-p65, p65, p-IκBα, IκBα, p-PTEN, and PTEN in the presence or absence of IPA/IAA mixture during the stimulation for 0–120 min at the protein level. β-actin was used as an internal control. K–P Quantitative analyses of p-p65, p65, p-IκBα, IκBα, p-PTEN, and PTEN expression. Q Representative images of the intracellular location of the NF-κB p65. R MH7A cells were cultured with or without the IPA/IAA mixture for 12 h and treated with cycloheximide (CHX) (50 μg/mL). Total cell lysates were subjected to immunoblotting analysis. S Quantitative analyses of PTEN expression treated with CHX in the presence or absence of IPA/IAA mixture (0, 4, 8, 12 h). T MH7A cells were pretreated with IPA/IAA mixture for 8 h, with or without MG132 (50 μM) for 2 h. The cell lysates were immunoprecipitated with PTEN antibody and immunoblotted with ubiquitin and PTEN antibodies. U–V Relative expression levels of ubiquitin in PTEN

Article Snippet: The primary antibody anti- NF-κB p65 (Cat#: bsm-33117 M-1, bioss, 1:1000), anti-Phospho-NFKB p65 (Ser276; Cat#: bs-3543R, bioss; 1:1000), anti-IkB-α (Cat#: BS3601, bioworld; 1:1000), anti-p-IkB-α (phospho-S32/S36; Cat#: BS4105, bioworld; 1:1000), anti-PTEN (Cat#: sc-7974, santa cruz; 1:1000), anti-p-PTEN (Cat#: sc-377573, santa cruz; 1:500) was added to the PVDF membranes and incubated overnight at 4 °C.

Techniques: Control, Expressing, Migration, Cell Culture, Western Blot, Immunoprecipitation, Ubiquitin Proteomics

Journal: Journal of Nanobiotechnology

Article Title: An orally-administered nanotherapeutics with gold nanospheres supplying for rheumatoid arthritis therapy by re-shaping gut microbial tryptophan metabolism

doi: 10.1186/s12951-025-03450-7

Figure Lengend Snippet: The IPA/IAA mixture exerted significant anti-inflammatory and protective effects in CIA mice. A Arthritis severity scores in CIA mice treated with the IPA/IAA mixture or normal saline. B Representative histological images of joint sections stained with H&E. C Representative TRAP-stained images of joint sections in CIA mice. D Representative photographs and micro-CT images of paws. E and F Representative immunohistochemical images showing expression of p-p65 and PTEN in fibroblast-like synoviocytes. G Histological scores of joints. H Quantitative analyses of TRAP staining. I Quantitative analyses of bone histomorphometry indexes. J and K The Average Optical Density (AOD) of p-p65 and PTEN

Article Snippet: The primary antibody anti- NF-κB p65 (Cat#: bsm-33117 M-1, bioss, 1:1000), anti-Phospho-NFKB p65 (Ser276; Cat#: bs-3543R, bioss; 1:1000), anti-IkB-α (Cat#: BS3601, bioworld; 1:1000), anti-p-IkB-α (phospho-S32/S36; Cat#: BS4105, bioworld; 1:1000), anti-PTEN (Cat#: sc-7974, santa cruz; 1:1000), anti-p-PTEN (Cat#: sc-377573, santa cruz; 1:500) was added to the PVDF membranes and incubated overnight at 4 °C.

Techniques: Saline, Staining, Micro-CT, Immunohistochemical staining, Expressing